Human TrkA PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1751RP

Recombinant Monoclonal Antibody
R&D Systems, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Flow Cytometry, Knockout Validated

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 165131R

View all TrkA Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TrkA
Ala33-Glu407
Accession # P04629

Specificity

Detects human TrkA in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TrkA PE-conjugated Antibody

Detection of TrkA antibody in BAF Cell Line Transfected with Human TrkA and eGFP antibody by Flow Cytometry.

Detection of TrkA in BAF Cell Line Transfected with Human TrkA and eGFP by Flow Cytometry.

BAF cell line transfected with human TrkA and eGFP was stained with (A) Mouse Anti-Human TrkA PE-conjugated Monoclonal Antibody (Catalog # FAB1751RP) or (B) Mouse IgG1 isotype control antibody (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Detection of TrkA antibody in K562 Human Cell Line antibody by Flow Cytometry.

Detection of TrkA in K562 Human Cell Line by Flow Cytometry.

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human TrkA PE-conjugated Monoclonal Antibody (Catalog # FAB1751RP, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
TrkA is specifically detected antibody in K562 human chronic myelogenous leukemia cell line parental cell line but is not detectable antibody in TrkA knockout K562 cell line.

TrkA is specifically detected in K562 human chronic myelogenous leukemia cell line parental cell line but is not detectable in TrkA knockout K562 cell line.

TrkA knockout K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human TrkA PE-connjugated Monoclonal Antibody (Catalog # FAB1751RP, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). No staining in the TrkA knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Applications for Human TrkA PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: BAF Cell Line Transfected with Human TrkA and eGFP and K562 human chronic myelogenous leukemia cell line

Knockout Validated

TrkA is specifically detected in K562 human chronic myelogenous leukemia cell line parental cell line but is not detectable in TrkA knockout K562 cell line.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TrkA

TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB, and TrkC preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGF R), a member of the tumor necrosis factor receptor superfamily. NGF R binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGF R can also mediate cell death when expressed independent of Trk.

Long Name

Neurotrophic Tyrosine Kinase Receptor A

Alternate Names

NTRK-1, NTRK1

Entrez Gene IDs

4914 (Human); 18211 (Mouse); 59109 (Rat); 102129364 (Cynomolgus Monkey)

Gene Symbol

NTRK1

UniProt

P04629

Additional TrkA Products

Product Documents for Human TrkA PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human TrkA PE-conjugated Antibody

For research use only

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