Detects human TrkB in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) TrkA, recombinant rat TrkA, recombinant mouse (rm) TrkB, rhTrkC, or rmTrkC is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human TrkB Alexa Fluor® 647-conjugated Antibody

Detection of TrkB in Human TrkB transfected C6 Cell Line by Flow Cytometry.

C6 cell line transfected with human TrkB was stained with Mouse Anti-Human TrkB Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB3971R, filled histogram) or isotype control antibody (IC002R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of TrkB in Baf3 cells by Flow Cytometry.

Baf3 cells transfected with Human TrkB (filled histogram) vs Irrelevant transfectant cells (open histogram) were stained with Mouse Anti-Human TrkB Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB3971R). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TrkB Alexa Fluor® 647-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

Titration recommended for optimal concentration with starting range of 0.1-1 µg/1 million cells. Samples used for this experiment were Human TrkB transfected C6 cells and Baf3 cells transfected with Human TrkB vs Irrelevant transfectant cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TrkB

The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and
NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.

References

Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
Soppet, D. et al. (1991) Cell 65:895.

Long Name

Neurotrophic Tyrosine Kinase Receptor B

Alternate Names

NTRK2

Entrez Gene IDs

4915 (Human); 18212 (Mouse); 25054 (Rat)

Gene Symbol

NTRK2

UniProt

Q16620

Additional TrkB Products

Product Documents for Human TrkB Alexa Fluor® 647-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human TrkB Alexa Fluor® 647-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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Human TrkB Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB3971R

Key Product Details

Species Reactivity

Validated:

Cited:

Applications