Human TRPS1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4838
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human TRPS1
Met1-Phe222
Accession # Q9UHF7
Met1-Phe222
Accession # Q9UHF7
Specificity
Detects human TRPS1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human TRPS1 Antibody
Detection of Human TRPS1 by Western Blot.
Western blot shows lysates of MDA-MB-453 human breast cancer cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRPS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4838) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRPS1 at approximately 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human TRPS1 by Simple WesternTM.
Simple Western lane view shows lysates of MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for TRPS1 at approximately 191 kDa (as indicated) using 10 µg/mL of Goat Anti-Human TRPS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4838) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.Applications for Human TRPS1 Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: MCF‑7 human breast cancer cell line
Sample: MCF‑7 human breast cancer cell line
Western Blot
1 µg/mL
Sample: MDA-MB-453 human breast cancer cell line and MCF-7 human breast cancer cell line
Sample: MDA-MB-453 human breast cancer cell line and MCF-7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF4838 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRPS1
TRPS1 (also GC79) is a 160-175 kDa (predicted 140 kDa) member of the zinc finger family of transcription factors. It is ubiquitously expressed and serves to repress the expression of GATA-regulated genes. Human TRPS1 is 1281 amino acids (aa) in length. It contains nine divergent zinc-finger motifs (aa 222‑1267), the seventh of which is associated with a GATA-binding sequence (aa 896‑920) and is flanked by two NLSs (aa 886‑891 and 946‑952). Two SUMOylation sites exist (Lys1192 and Lys1201) and when utilized, they each add 40 kDa to the native MW. One alternative splice form shows premature truncation after Lys853. A second shows an alternate start site 13 aa upstream of the standard start site. Over aa 1‑222, human TRPS1 shares 95% and 89% aa identity with canine and mouse TRPS1, respectively.
Long Name
Tricho-rhino-phalangeal Syndrome Type I Protein
Alternate Names
GC79
Gene Symbol
TRPS1
UniProt
Additional TRPS1 Products
Product Documents for Human TRPS1 Antibody
Product Specific Notices for Human TRPS1 Antibody
For research use only
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