Human Trypsin 2/PRSS2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3586
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 410322
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Trypsin 2/PRSS2
Ala16-Ser247
Accession # P07478
Ala16-Ser247
Accession # P07478
Specificity
Detects human Trypsin 2/PRSS2 in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) Trypsin 1 or rhTrypsin 3. Recognizes both the pro and mature forms of rhTrypsin 2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Trypsin 2/PRSS2 Antibody
Detection of Human Trypsin 2/PRSS2 by Western Blot.
Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Trypsin 2/PRSS2 Monoclonal Antibody (Catalog # MAB3586) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Trypsin 2/PRSS2 at approximately 24 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.
Detection of Human Trypsin 2/PRSS2 by Western Blot.
Western blot shows recombinant human Trypsin 1, recombinant human Trypsin 2 and recombinant human Trypsin 3. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Trypsin-2/PRSS2 Monoclonal Antibody (Catalog # MAB3586) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF108). A specific band was detected for Trypsin-2/PRSS2 at approximately 24 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.
Detection of Human Trypsin 2/PRSS2 by Immunoprecipitation.
Human Trypsin 2/PRSS2 was immunoprecipitated from 500 μg of human pancreas lysates with 12.5 ug Mouse Anti-Human Trypsin 2/PRSS2 Monoclonal Antibody (Catalog # MAB3586). The Trypsin 2/PRSS2-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated human Trypsin 2/PRSS2 was detected by Western blot using 2 µg/mL of Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody (AF3586) under non-reducing conditions and using Western Blot Buffer Group 1.Applications for Human Trypsin 2/PRSS2 Antibody
Application
Recommended Usage
Immunoprecipitation
12.5 µg/500 µg cell lysate
Sample: Human pancreas tissue
Sample: Human pancreas tissue
Western Blot
2 µg/mL
Sample: Human Pancreas Tissue and Recombinant Human Trypsin 2/PRSS2 under non-reducing conditions only
Sample: Human Pancreas Tissue and Recombinant Human Trypsin 2/PRSS2 under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4 using MAB3586 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Trypsin 2/PRSS2
Human Trypsin 2, encoded by the PRSS2 gene, is also known as anionic Trypsin (1). Accounting for approximately 1/3 of the total trypsin content in the pancreatic juice, it is one of the trypsins produced in the pancreas. It consists of a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 2 is synthesized as a zymogen and secreted into the duodenal lumen, where it is activated by enteropeptidase (2). It plays a central role in digestion and activating other pro-enzymes. In human pancreatic diseases and chronic alcoholism, it is up-regulated (3).
References
- Emi, M. et al. (1986) Gene. 41:305.
- Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 1483-1488, Academic Press, San Diego.
- Rinderknecht, H. et al. (1979) Gut. 20:886.
Alternate Names
PRSS2, Tesp4, TRY8, Trypsinogen 20
Gene Symbol
PRSS2
UniProt
Additional Trypsin 2/PRSS2 Products
Product Documents for Human Trypsin 2/PRSS2 Antibody
Product Specific Notices for Human Trypsin 2/PRSS2 Antibody
For research use only
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