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Human Tryptase  alpha/TPS1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2370

R&D Systems, part of Bio-Techne
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MAB2370
MAB2370-SP

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 274001

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Tryptase alpha/TPS1
Ile31-Pro275
Accession # AAA86934

Specificity

Detects human Tryptase alpha/TPS1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50‑100% cross‑reactivity with recombinant human (rh) Tryptase beta2 and no cross-reactivity with rhTryptase gamma1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Tryptase  alpha/TPS1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Tryptase  alpha/TPS1, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Tryptase  alpha/TPS1
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase alpha/TPS1

Tryptase alpha is a neutral serine protease that is enriched in mast cell granules. It has a unique Asp residue in the substrate-binding region and has either very restricted specificity or no protease activity at all.

Alternate Names

alpha-Tryptase, TPS1, TPSAB1, Tryptase 1

Entrez Gene IDs

7177 (Human)

Gene Symbol

TPSAB1

UniProt

Additional Tryptase alpha/TPS1 Products

Product Documents for Human Tryptase  alpha/TPS1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Tryptase  alpha/TPS1 Antibody

For research use only

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