Human Tryptase epsilon/BSSP-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5047
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 725445
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Tryptase epsilon/BSSP-4
Ala33-Ser317
Accession # Q9GZN4
Ala33-Ser317
Accession # Q9GZN4
Specificity
Detects human Tryptase epsilon/BSSP-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Tryptase epsilon/BSSP-4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Tryptase epsilon/BSSP-4 Antibody
Detection of Human Tryptase epsilon/BSSP‑4 by Western Blot.
Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Tryptase e/BSSP-4 Monoclonal Antibody (Catalog # MAB5047) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tryptase e/BSSP-4 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Tryptase epsilon/BSSP‑4 in Human Pancreas.
Tryptase e/BSSP-4 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Tryptase e/BSSP-4 Monoclonal Antibody (Catalog # MAB5047) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Tryptase epsilon/BSSP-4 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Western Blot
2 µg/mL
Sample: Human pancreas tissue
Sample: Human pancreas tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tryptase epsilon/BSSP-4
Tryptase epsilon, also known as brain-specific protease-4 (BSSP-4) and prosemin, is expressed in the brain and epithelium-rich tissues such as the lung and eye (1, 2). BSSP-4 is a serine protease related to trypsin, preferentially hydrolyzing substrates after arginine and lysine residues. However, Tryptase epsilon is less susceptible to inhibition by common trypsin inhibitors such as aprotinin, alpha1-antitrypsin and secretory leukocyte protease inhibitor (3). Tryptase epsilon efficiently converts pro-urokinase-type plasminogen activator to its mature, active form (3).
References
- Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.
- Wong, G.W. et al. (2001) J. Biol. Chem. 276:49169.
- Yasuda, S. et al. (2005) Blood 105:3893.
Alternate Names
BSP-2, BSSP-4, BSSP4, PRSS22
Gene Symbol
PRSS22
UniProt
Additional Tryptase epsilon/BSSP-4 Products
Product Documents for Human Tryptase epsilon/BSSP-4 Antibody
Product Specific Notices for Human Tryptase epsilon/BSSP-4 Antibody
For research use only
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