Western blot shows lysates of NS0 mouse myeloma cell line mock transfected or transfected with human TSLP R. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human TSLP R Monoclonal Antibody (Catalog # MAB9811) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). Specific bands were detected for TSLP R at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of TSLPR in NS0 cells transfected with hTSLPR vs Irrelevant NS0 transfectant cells by Flow Cytometry.

NS0 cells transfected with hTSLPR (filled histogram) vs Irrelevant NS0 transfectant cells (open histogram) were stained with Mouse Anti-Human TSLPR Monoclonal Antibody (Catalog # MAB9811) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TSLPR Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: NS0 cells transfected with hTSLPR vs Irrelevant NS0 transfectant cells

Western Blot

0.5 µg/mL
Sample: NS0 mouse myeloma cell line transfected with human TSLP R

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSLPR

TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The human TSLP R cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS-like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLP R displays 39% identity to mouse TSLP R and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported in mouse (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3‑6). The TSLP R and IL-7 R alpha are co‑expressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell-attracting chemokines from monocytes and enhance the maturation of CD11c⁺ dendritic cells (5).

References

Fujio, K. et al. (2000) Blood 95:2204.
Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
Park, L.S. et al. (2000) J. Exp. Med. 192:659.
Tonozuka, Y. et al. (2001) Cytogenet. Cell Genet. 93:23.
Reche, P.A. et al. (2001) J. Immunol. 167:336.
Pandey, A. et al. (2000) Nat. Immunol. 1:59.

Long Name

Thymic Stromal Lymphopoietin Receptor

Alternate Names

CRL2, CRLF2, CRLM-2, PCOR1, TSLP R

Entrez Gene IDs

64109 (Human); 57914 (Mouse); 171499 (Rat)

Gene Symbol

CRLF2

UniProt

Q9HC73

Additional TSLPR Products

Product Documents for Human TSLPR Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TSLPR Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TSLPR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9811

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all TSLPR Antibodies »

Product Summary for Human TSLPR Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TSLPR Antibody

Detection of Human TSLP R by Western Blot.