Human TSP50 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2430
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TSP50
Ala40-Ala363
Accession # Q9UI38
Ala40-Ala363
Accession # Q9UI38
Specificity
Detects human TSP50 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse TSP50 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human TSP50 Antibody
Detection of Human TSP50 by Western Blot.
Western blot shows lysates of human testis tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TSP50 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2430) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TSP50 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human TSP50 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human TSP50, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human TSP50, see our available Western blot detection antibodies
Western Blot
0.2 µg/mL
Sample: Human testis tissue
Sample: Human testis tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TSP50
Long Name
Testes-specific Protease 50
Alternate Names
Cancer/testis antigen 20, CT20probable threonine protease PRSS50, EC 3.4.25.-, protease, serine, 50, Serine protease 50, testes-specific protease 50, Testis-specific protease-like protein 50, TSP50testes specific protease 50
Gene Symbol
PRSS50
UniProt
Additional TSP50 Products
Product Documents for Human TSP50 Antibody
Product Specific Notices for Human TSP50 Antibody
For research use only
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