Human TXNDC5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4840
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human TXNDC5
Glu110-Leu432
Accession # Q8NBS9
Glu110-Leu432
Accession # Q8NBS9
Specificity
Detects endogenous human TXNDC5 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human TXNDC5 Antibody
Detection of Human TXNDC5 by Western Blot.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human TXNDC5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4840) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TXNDC5 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human TXNDC5 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HT-29 human colon adenocarcinoma cell line, A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line
Sample: HT-29 human colon adenocarcinoma cell line, A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line
Reviewed Applications
Read 1 review rated 4 using AF4840 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TXNDC5
TXNDC5 (Thioredoxin (TRX) domain-contain protein 5; also ERp46 and EndoPDI) is a 50‑55 kDa member of the disulfide isomerase family of enzymes. It is expressed in hepatocytes, and tumor plus hypoxic endothelium. TXNDC5 demonstrates thioreductase (disulfide bond reduction) activity in the ER. Mature human TXNDC5 is 400 amino acids (aa) in length and migrates anomalously in SDS-PAGE. It contains three TRX domains (aa 36‑428) and a short ER retention motif (aa 429‑432). There are three potential splice variants. One shows an alternate start site at Met109, while two others show a 16 and 45 aa substitution for the N-terminal 88 amino acids. Over aa 110‑432, human TXNDC5 is 88% aa identical to mouse TXNDC5.
Long Name
Thioredoxin Domain Containing 5
Alternate Names
EndoPDI, ERp46, TLP46
Gene Symbol
TXNDC5
UniProt
Additional TXNDC5 Products
Product Documents for Human TXNDC5 Antibody
Product Specific Notices for Human TXNDC5 Antibody
For research use only
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