Detects human ULBP-2 and human RAET1L/ULBP-6 in direct ELISA and stains cells transfected with human ULBP-2, human ULBP-5 or human RAET1L/ULBP-6 in flow cytometry. In direct ELISA, 11% cross‑reactivity with recombinant human ULBP5 is observed. It does not stain cells transfected with human ULBP-1 or human ULBP-3 in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human ULBP-2/5/6 Alexa Fluor® 647-conjugated Antibody

Detection of ULBP-2/5/6 in HT1080 Human Cell Line by Flow Cytometry.

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB1298R, filled histogram) or isotype control antibody (Catalog # IC003R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human ULBP-2/5/6 Alexa Fluor® 647-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

0.25-1 µg/10⁶ cells
Sample: HT1080 human fibrosarcoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: ULBP-2/5/6

ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. The name ULBP derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes for ULBPs reside in a cluster of ten related genes, six of which encode potentially functional glycoproteins. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5 also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are distantly related to MHC class I proteins, but they possess only the alpha1 and alpha2 Ig-like domains, and they have no capacity to bind peptide or interact with beta2‑microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and 95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.

References

Cosman, D. et al. (2001) Immunity 14:123.
Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
Sutherland, C. et al. (2002) J. Immunol. 168:671.
Steinle, A. et al. (2001) Immunogenetics 53:279.
Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
Pende, D. et al. (2002) Cancer Res. 62:6178.
Radosavljevic, M. et al. (2002) Genomics 79:114.
NKG2D and its Ligands (2002) www.RnDSystems.com.

Long Name

UL16 Binding Protein-2/5/6

Alternate Names

ALCAN-alpha, N2DL2, NKG2DL2, RAET1H, RAET1L, UL16 binding protein 2, ULBP2

UniProt

Q9BZM5

Additional ULBP-2/5/6 Products

Product Documents for Human ULBP-2/5/6 Alexa Fluor® 647-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human ULBP-2/5/6 Alexa Fluor® 647-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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Human ULBP-2/5/6 Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1298R

Key Product Details

Species Reactivity

Validated:

Cited:

Applications