Human UNC13B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6188

R&D Systems, part of Bio-Techne
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MAB6188
MAB6188-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 584102

View all UNC13B Antibodies »

Product Summary for Human UNC13B Antibody

Immunogen

E. Coli-derived recombinant human UNC13B
Asp418-Ser1591
Accession # O14795

Specificity

Detects human UNC13B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) UNC13A and rhUNC13C is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human UNC13B Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion-fixed paraffin-embedded sections of human kidney and human kidney cancer tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UNC13B

UNC13B is a 170 kDa member of the unc13 family. It is 1591 amino acids (aa) in length and contains three C2 domains, one MHD1 domain, one MHD2 domain, and one phorbol-ester/DAG-type zinc finger. Human UNC13B is 95% and 93% aa identical to mouse and rat UNC13B, respectively. UNC13B is expressed in brain and kidney cortical epithelial and mesangial cells. UNC13B plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. It is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-depending refilling of readily releasable vesicle pool (RRP). In addition, UNC13B may be involved in mediating some of the acute and chronic changes in mesangial cells produced by exposure to hyperglycemia

Long Name

UNC-13 Homolog B

Alternate Names

hmunc13, MUNC13B, Unc13h2

Entrez Gene IDs

10497 (Human)

Gene Symbol

UNC13B

UniProt

O14795

Additional UNC13B Products

Product Documents for Human UNC13B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human UNC13B Antibody

For research use only

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