Human UNC13D Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB89661
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1223A
Product Specifications
Immunogen
KLH-coupled N-terminal human UNC13D peptide
Accession # Q70J99
Accession # Q70J99
Specificity
Detects the conventional form of human UNC13D in Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human UNC13D Antibody
Detection of Human UNC13D by Western Blot.
Western blot shows lysates of NK-92 human natural killer lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB89661) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for UNC13D at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.UNC13D in NK‑92 Human Cell Line.
UNC13D was detected in immersion fixed NK-92 human natural killer lymphoma cell line using Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB89661) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Detection of Human UNC13D by Simple WesternTM.
Simple Western lane view shows lysates of NK‑92 human natural killer lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for UNC13D at approximately 112 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB89661). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human UNC13D Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed NK‑92 human natural killer lymphoma cell line
Sample: Immersion fixed NK‑92 human natural killer lymphoma cell line
Simple Western
1 µg/mL
Sample: NK‑92 human natural killer lymphoma cell line
Sample: NK‑92 human natural killer lymphoma cell line
Western Blot
0.1 µg/mL
Sample: NK‑92 human natural killer lymphoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, and Ramos human Burkitt's lymphoma cell line
Sample: NK‑92 human natural killer lymphoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, and Ramos human Burkitt's lymphoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB89661 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: UNC13D
Long Name
UNC-13 Homolog D
Alternate Names
FHL3, HLH3, HPLH3, Munc13-4
Gene Symbol
UNC13D
UniProt
Additional UNC13D Products
Product Documents for Human UNC13D Antibody
Product Specific Notices for Human UNC13D Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...