I'm looking Munc13-4 N terminal antibody with related epitope sequence.

Name: Human UNC13D Antibody
Brand: R&D Systems
SKU: MAB89661
Rating: 5 (1 reviews)

Unfortunately we do not epitope map our antibodies, but we do have a whole list of antibodies against your target depending on species reactivity and application you want to use them for. Please see this link to our MUNC 13-4 antibodies. All of the immunogen information that is not proprietary, or known should be presented on the datasheet. Often times a range will be provided where the peptide falls within, or if it was raised against a larger recombinant protein as is the case for the one you inquired about we do not know exactly where the binding occurs.

Human UNC13D Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB89661

(1)

Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody.

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1223A

View all UNC13D Antibodies »

Product Summary for Human UNC13D Antibody

Immunogen

KLH-coupled N-terminal human UNC13D peptide
Accession # Q70J99

Specificity

Detects the conventional form of human UNC13D in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human UNC13D Antibody

Detection of Human UNC13D by Western Blot.

Western blot shows lysates of NK-92 human natural killer lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB89661) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for UNC13D at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

UNC13D in NK‑92 Human Cell Line.

UNC13D was detected in immersion fixed NK-92 human natural killer lymphoma cell line using Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB89661) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human UNC13D by Simple Western^TM.

Simple Western lane view shows lysates of NK‑92 human natural killer lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for UNC13D at approximately 112 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB89661). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human UNC13D Antibody

Application

Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed NK‑92 human natural killer lymphoma cell line

Simple Western

1 µg/mL
Sample: NK‑92 human natural killer lymphoma cell line

Western Blot

0.1 µg/mL
Sample: NK‑92 human natural killer lymphoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, and Ramos human Burkitt's lymphoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB89661 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UNC13D

UNC13D (also Munc13-4) is a 123 kDa cytoplasmic and peripheral membrane protein that is expressed at highest levels in hematopoietic tissues. UNC13D appears to play a role in vesicle maturation during exocytosis and its expression is obligatory for exocytosis of cytolytic granules from NK and T cells. A point mutation in intron 1 of UNC13D causes familial hematophagocytic lymphohistiocytosis type3, a rare autosomal recessive immune deficiency. The mutation disrupts transcription factor binding and prevents expression of an alternative isoform that is required for lymphocyte cytotoxicity. The conventional and alternative isoforms are identical from amino acids (aa) 40-1090 but have distinct N-terminal segments. Human and mouse have 75% aa sequence identity throughout the region used as the immunogen. It is unknown whether mice express the alternative isoform.

Long Name

UNC-13 Homolog D

Alternate Names

FHL3, HLH3, HPLH3, Munc13-4

Entrez Gene IDs

201294 (Human); 70450 (Mouse); 192177 (Rat)

Gene Symbol

UNC13D

UniProt

Q70J99

Additional UNC13D Products

Product Documents for Human UNC13D Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human UNC13D Antibody

For research use only

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