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Human Vasorin/SLIT-like 2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2140

R&D Systems, part of Bio-Techne
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MAB2140
MAB2140-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

ELISA Development, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 227526

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Vasorin
aa 24-575
Accession # Q6EMK4

Specificity

Detects human Vasorin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Vasorin/SLIT-like 2 Antibody

Detection of Human Vasorin/SLIT-like 2 antibody by Western Blot.

Detection of Human Vasorin/SLIT‑like 2 by Western Blot.

Western blot shows lysates of human placenta tissue and human lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Vasorin/SLIT-like 2 Monoclonal Antibody (Catalog # MAB2140) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Vasorin/SLIT-like 2 at approximately 65 and 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Vasorin/SLIT-like 2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human placenta tissue and human lung tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vasorin/SLIT-like 2

Vasorin is a type I membrane protein that is predominantly expressed in vascular smooth muscle cells in a developmentally regulated pattern. Expression is down-regulated during vessel repair after arterial injury. Vasorin directly binds to transforming growth factor (TGF)-beta and attenuates TGF-beta signaling in vitro (1).

References

  1. Ikeda, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10732.

Alternate Names

Atia, SLIT like 2, Slitl2, VASN

Entrez Gene IDs

114990 (Human); 246154 (Mouse)

Gene Symbol

VASN

UniProt

Additional Vasorin/SLIT-like 2 Products

Product Documents for Human Vasorin/SLIT-like 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Vasorin/SLIT-like 2 Antibody

For research use only

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