Human VIGR/GPR126 MAb (Clone 1044430)

R&D Systems, part of Bio-Techne | Catalog # MAB10577

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1044430

View all VIGR/GPR126 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived human VIGR/GPR126 protein
Cys38-Lys437
Accession # AAH75798.1

Specificity

Detects human VIGR/GPR126 protein in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human VIGR/GPR126 MAb (Clone 1044430)

Detection of VIGR/GPR126 in HEK293 Human Cell Line Transfected with Human VIGR/GPR126 by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human VIGR/GPR126 (filled histogram) or irrelevant protein (open histogram) was stained with Mouse Anti-Human VIGR/GPR126 Monoclonal Antibody (Catalog # MAB10577) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Gate was set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human VIGR/GPR126 MAb (Clone 1044430)

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human VIGR/GPR126

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VIGR/GPR126

VIGR (Vascular Inducible G Protein-coupled Receptor), also known as ADGRG6, DREG, and GPR126, is a neuronal 7 TM pass (G protein)-coupled receptor (GPCR) involved in myelination and glial and Schwann cell development (1, 2). Human VIGR cDNA encodes a 1221 amino acid (aa) residue membrane protein with a 37 aa signal peptide, a 825 aa extracellular domain (ECD) with 27 potential N-linked glycosylation sites, seven transmembrane segments that span between aa 863 and aa 1113, and a 108 aa residue cytoplasmic domain. Within ECD human VIGR shares 83% aa sequence identity with mouse and rat VIGR. VIGR is essential for the development of diverse organs (1, 2). Type IV collagen, a major constituent of the basement membrane, binds to VIGR and activates its signaling function (3). This interaction stimulated the production of cAMP in rodent Schwann cells, which require VIGR activity to differentiate, and in human embryonic kidney (HEK293) cells expressing exogenous VIGR. Laminin-211 binds a novel laminin-binding domain in VIGR N-terminal fragment between aa 446 and 807 (4). VIGR-Laminin-211 interactions regulate terminal differentiation and myelination by ensuring appropriate levels of cAMP for a given stage of Schwann cell development (4).

References

  1. Rughetti, A. et al. (2005) J. Immunol. 174:7764.
  2. Engelstaedter, V. et al. (2012) BMC Cancer 12:600.
  3. Taylor-Papadimitriou, J. et al. (1999) Biochim. Biophys. Acta 1455:301.
  4. Geng, Y. et al. (2012) Front Oncol. 2:76.
  5. Tanida, S. et al. (2013) J Biol Chem. 288:31842.
  6. Beatson, R. et al. (2016) Nat Immunol. 17:1273.
  7. Piyush, T. et al. (2017) Cell Death Differ. 24:1937.

Long Name

Vascular-inducible G Protein-coupled Receptor

Alternate Names

APG1, DREG, Gm222, GPR126, PS1TP2

Entrez Gene IDs

57211 (Human); 215798 (Mouse); 308376 (Rat)

Gene Symbol

ADGRG6

UniProt

AAH75798.1

Additional VIGR/GPR126 Products

Product Documents for Human VIGR/GPR126 MAb (Clone 1044430)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human VIGR/GPR126 MAb (Clone 1044430)

For research use only

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