Human VSIG3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB92291
Key Product Details
Species Reactivity
Human
Applications
Blockade of Receptor-ligand Interaction
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 774206
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VSIG3
Met1-Gly245
Accession # Q5DX21
Met1-Gly245
Accession # Q5DX21
Specificity
Detects human VSIG3 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human VSIG3 Antibody
VSIG3 Binding to VISTA/B7-H5/PD-1H by Human VISG3 Antibody.
In a functional ELISA, 0.2-2 µg/mL of this antibody will block 50% of the binding of 1 µg/mL of Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS) to immobilized Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (Catalog # 7126-B7) coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding.Applications for Human VSIG3 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.2-2 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS ) to immobilized Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (Catalog # 7126-B7) coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VSIG3
VSIG3 (V-set and Ig domain-containing protein 3; also BT-IgSF and IGSF11) is a 52 kDa brain and testis-specific protein that belongs to the IGSF11 family of proteins. It is expressed by neurons, astrocytes and oligodendroglia. VSIG3 is an adhesion molecule that forms Ca-independent homophilic interactions in trans. Human VSIG3 is 413 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 219 aa extracellular domain (ECD). The ECD contains one V-type (aa 23‑136) and one C2-type Ig-like domain (aa 144‑234). Over aa 23‑245, human VSIG3 is 94% aa identical to mouse VSIG3. Two potential splice variants exist in human. Both exhibit a 16 aa substitution for the first 17 aa of the signal sequence, and one contains an additional single Ala substitution for aa 211‑235.
Long Name
V-Set and Immunoglobulin Domain Containing 3
Alternate Names
BTIGSF, CXADRL1, IGSF11
Gene Symbol
IGSF11
UniProt
Additional VSIG3 Products
Product Documents for Human VSIG3 Antibody
Product Specific Notices for Human VSIG3 Antibody
For research use only
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