Human VSIG3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB92293
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Blockade of Receptor-ligand Interaction, CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 973422
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VSIG3
Met1-Gly245
Accession # Q5DX21
Met1-Gly245
Accession # Q5DX21
Specificity
Detects human VSIG3 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human VSIG3 Antibody
Detection of VSIG3 in HEK293 Human Cell Line transfected with human VSIG3 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human VSIG3 or (B) irrelevant protein, and eGFP, was stained with Mouse anti-Human VSIG3 monoclonal antibody (Catalog # MAB92293) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on isotype control antibody staining (MAB0041, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.
VSIG3 Binding to VISTA/B7-H5/PD-1H by Human VISG3 Antibody.
In a functional ELISA, 0.2-2 µg/mL of Mouse Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB92293) will block 50% of the binding of 1 µg/mL of Recombinant Human VSIG3 Fc Chimera (9229-VS) to immobilized Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (7126-B7) coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding.Applications for Human VSIG3 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.2-2 µg/mL of this antibody will block 50% of the binding of 1 µg/mL of Recombinant Human VSIG3 Fc Chimera to immobilized Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line transfected with human VSIG3 and eGFP
Sample: HEK293 Human Cell Line transfected with human VSIG3 and eGFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VSIG3
VSIG3 (V-set and Ig domain-containing protein 3; also BT-IgSF and IGSF11) is a 52 kDa brain and testis-specific protein that belongs to the IGSF11 family of proteins. It is expressed by neurons, astrocytes and oligodendroglia. VSIG3 is an adhesion molecule that forms Ca-independent homophilic interactions in trans. Human VSIG3 is 413 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 219 aa extracellular domain (ECD). The ECD contains one V-type (aa 23‑136) and one C2-type Ig-like domain (aa 144‑234). Over aa 23‑245, human VSIG3 is 94% aa identical to mouse VSIG3. Two potential splice variants exist in human. Both exhibit a 16 aa substitution for the first 17 aa of the signal sequence, and one contains an additional single Ala substitution for aa 211‑235.
Long Name
V-Set and Immunoglobulin Domain Containing 3
Alternate Names
BTIGSF, CXADRL1, IGSF11
Gene Symbol
IGSF11
UniProt
Additional VSIG3 Products
Product Documents for Human VSIG3 Antibody
Product Specific Notices for Human VSIG3 Antibody
For research use only
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