Human VSIG8 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB9418P

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 961823

View all VSIG8 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived transfected with human VSIG8
Met1-Gly263
Accession # Q5VU13

Specificity

Detects human VSIG8 in direct ELISAs. Stains human VSIG8 transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human VSIG8 PE-conjugated Antibody

Detection of VSIG8 antibody in HEK293 Human Cell Line Transfected with Human VSIG8 and eGFP antibody by Flow Cytometry.

Detection of VSIG8 in HEK293 Human Cell Line Transfected with Human VSIG8 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human VSIG8 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human VSIG8 PE-conjugated Monoclonal Antibody (Catalog # FAB9418P). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human VSIG8 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 Human Cell Line Transfected with Human VSIG8 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VSIG8

VSIG8 (V-set and immunoglobulin domain containing 8), also known as C1orf204, is an approximately 45 kDa type I transmembrane protein of the B7 family within the Ig superfamily. Mature human VSIG8 consists of a 242 amino acid (aa) extracellular domain (ECD) containing two V-type Ig-like domains, a 21 aa transmembrane domain, and a 130 aa cytoplasmic domain. Within the ECD, human VSIG8 shares 88% and 89% aa identity with mouse and rat VSIG8, respectively. Alternative splicing generates a long isoform of human VSIG8 with a substitution in the cytoplasmic juxtamembrane region and a 124 aa extension at the C-terminus. VSIG8 was identified from proteomic analysis of human hair shafts (1, 2). It is expressed in the hair follicle and shaft, superficial layers of the nail matrix, and superficial layers of oral epithelium (3). R&D Systems in-house testing indicates that VSIG8 inhibits the production of IL-2 by activated T cells.

References

  1. Rice, R.H. et al. (2010) J. Proteome Res. 9:6752.
  2. Lee, Y.J. et al. (2006) Mol. Cell. Proteomics 5:789.
  3. Rice, R.H. et al. (2011) J. Invest. Dermatol. 131:1936.

Long Name

V-Set and Immunoglobulin Domain Containing 8

Alternate Names

V-set and immunoglobulin domain containing 8

Entrez Gene IDs

391123 (Human); 240916 (Mouse); 289236 (Rat); 102123183 (Cynomolgus Monkey)

Gene Symbol

VSIG8

UniProt

Q5VU13

Additional VSIG8 Products

Product Documents for Human VSIG8 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human VSIG8 PE-conjugated Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.