HEK293 human embryonic kidney cell line transfected with either (A) human VSTM2A or (B) irrelevant protein, and eGFP, was stained with Mouse anti-human VSTM2A monoclonal antibody (Catalog # MAB100371) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB10041, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Human VSTM2A ELISA Standard Curve.

Recombinant Human VSTM2A protein was serially diluted 2-fold and captured by Mouse Anti-Human VSTM2A Monoclonal Antibody (MAB10037) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human VSTM2A Monoclonal Antibody (Catalog # MAB100371) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Applications for Human VSTM2A Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human VSTM2A Monoclonal Antibody (Catalog # MAB10037).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line transfected with Human VSTM2A and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSTM2A

V-set and transmembrane domain-containing protein 2A (VSTM2A) is a secreted glycoprotein that is expressed by committed preadipocytes. N-linked glycosylation is crucial for its secretion, but not for preadipocyte cell differentiation activity. It is expressed during adipocyte development and its over-expression promotes adipogenesis (1). VSTM2A is highly expressed in the brain and Vstm2a was identified as an enigmatic gene that is highly produced in mouse brain (1, 2). A positive association has been observed between Vstm2a and Pparg2. VSTM2A plays a role in the regulation of the early stage of white and brown preadipocyte cell differentiation. It promotes adipogenic commitment of preadipocytes by increasing gene expression of the transcription factor PPARG in a BMP4-dependent signaling pathway (1, 3). In humans, two isoforms (1 and 2) exist due to alternative splicing. Human VSTM2A is synthesized either as a 236 amino acid (aa) (Isoform 1) or a 244 aa (Isoform 2) precursor that contains a 24 aa signal sequence followed by the VSTM2A domain. Human Isoform 2 VSTM2A shares 78% aa sequence identity with mouse and rat VSTM2A.

References

Secco, B. et al. (2017) Cell Rep. 18:93.
Pandey, A.K. et al. (2014) PloS One. 9:e88889.
Berry, D.C. et al. (2013) Development. 140:3939.

Long Name

V-Set And Transmembrane Domain Containing 2A

Alternate Names

VSTM2

Entrez Gene IDs

222008 (Human); 211739 (Mouse); 689106 (Rat)

Gene Symbol

VSTM2A

UniProt

Q8TAG5-2

Additional VSTM2A Products

Product Documents for Human VSTM2A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human VSTM2A Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human VSTM2A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB100371

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all VSTM2A Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human VSTM2A Antibody

Detection of VSTM2A in HEK293 Human Cell Line transfected with Human VSTM2A and eGFP by Flow Cytometry