Human WIF-1 Biotinylated Antibody

Name: Human WIF-1 Biotinylated Antibody
Brand: R&D Systems
SKU: BAM1341

R&D Systems, part of Bio-Techne | Catalog # BAM1341

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Product Datasheet / COA / SDS

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA Detection (Matched Antibody Pair), Western Blot

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ Clone # 133016

View all WIF-1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human WIF-1
Gly29-Trp379
Accession # AAD25402

Specificity

Detects human WIF-1 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity with recombinant mouse WIF-1 or recombinant human Wnt-7a is observed. In Western blots, 50% cross-reactivity with recombinant mouse WIF-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Human WIF-1 Biotinylated Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human WIF-1 (Catalog # 1341-WF)

Human WIF-1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:

Capture Reagent: Human WIF-1 Antibody (Catalog # MAB1342)
Standard: Recombinant Human WIF-1 Protein (Catalog # 1341-WF)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WIF-1

WIF (Wnt Inhibitory Factor) is a secreted protein that binds to Wnt proteins and inhibits their activity. The protein is synthesized as a 379 amino acid (aa) molecule that contains an N-terminal signal sequence, 150 aa WIF domain, 5 EGF-like repeats, and a hydrophilic domain at the carboxy terminus (1). In situ hybridization analysis from the frog, Xenopus laevis, and zebrafish indicate that the message is highly expressed in presomitic mesoderm, the notochord, anterior regions of the brain, branchial arches, nasal placodes, and otic vescicles (1). WIF inhibits secondary axis induction by Wnts and promotes secondary axis induction by Chordin in Xenopus embryos (1). In vitro, WIF binds to Drosophila Wingless and Xenopus Wnt‑8 proteins (1). WIF-1 is implicated as an early event tumor suppressor in cancers of the prostate, breast, lung and bladder (2). However, WIF-1’s role in carcinogenesis may not be that simple since in other cancer types, such as colon adenocarcinoma, WIF facilitates tumorigenesis (3). Human WIF-1 shares 94% and 82% amino acid identity with mouse and frog, respectively.

References

Hsieh, J-C. et al. (1999) Nature 398:431.
Wissmann, C. et al. (2003) J. Pathol 201:204.
Cebrat, M. et al. (2004) Cancer Lett. 206:107.

Long Name

Wnt Inhibitory Factor

Alternate Names

WIF1

Entrez Gene IDs

11197 (Human); 24117 (Mouse)

Gene Symbol