Human XPA Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3416
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Bioassay
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human XPA
Met1-Met273
Accession # P23025
Met1-Met273
Accession # P23025
Specificity
Detects human XPA in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human XPA Antibody
Detection of Human XPA by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3416) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPA at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 5.Applications for Human XPA Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XPA
The Xeroderma Pigmentosum A (XPA) protein is a component of a large complex that binds to damaged DNA to facilitate nucleotide excision repair (NER). XPA binds in a complex with Replication Protein A (RPA), XPG, and TFIIH (a subcomplex of the RNA polymerase II transcription initiation complex). XPA is thought to stabilize the binding of RPA to DNA, and thus aid in specific recognition of the damaged base. Because of their NER defect, XP patients exhibit hypersensitivity to ultraviolet light and have a heightened incidence of skin cancer.
Long Name
Xeroderma Pigmentosum, Complementation Group A
Alternate Names
XP1
Gene Symbol
XPA
UniProt
Additional XPA Products
Product Documents for Human XPA Antibody
Product Specific Notices for Human XPA Antibody
For research use only
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