Human XPE/DDB2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3297
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human XPE/DDB2
Met1-Lys427
Accession # Q92466
Met1-Lys427
Accession # Q92466
Specificity
Detects human XPE/DDB2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human XPE/DDB2 Antibody
Detection of Human XPE/DDB2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPE/DDB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3297) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPE/DDB2 at approximately 45 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human XPE/DDB2 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and U2OS human osteosarcoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line and U2OS human osteosarcoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XPE/DDB2
Xeroderma Pigmentosum complementation group E (XPE) patients are defective in a heterodimer of damage-specific DNA-binding activity (DDB1/DDB2). DDB2 is a 48 kDa protein upregulated in cells exposed to ultraviolet light. XP patients exhibit hypersensitivity to ultraviolet light and have a heightened incidence of skin cancer.
Long Name
Xeroderma Pigmentosum E
Alternate Names
DDB2, XP5
Gene Symbol
DDB2
UniProt
Additional XPE/DDB2 Products
Product Documents for Human XPE/DDB2 Antibody
Product Specific Notices for Human XPE/DDB2 Antibody
For research use only
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