Human XPV Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3417
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human XPV
Met1-Phe400
Accession # Q9Y253
Met1-Phe400
Accession # Q9Y253
Specificity
Detects human XPV in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human XPV Antibody
Detection of Human XPV by Western Blot.
Western blot shows lysates of MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPV Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3417) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPV at approximately 80 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 5.Applications for Human XPV Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: MRC-5 human embryonic lung fibroblast cell line
Sample: MRC-5 human embryonic lung fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XPV
Xeroderma pigmentosum variant (XPV) is a form of XP caused by mutation in the DNA polymerase eta (POLH) gene. DNA polymerase eta catalyzes translesion DNA synthesis past pyrimidine dimers, a mutation caused by exposure to ultraviolet (UV) light. XPV patients are sensitive to UV light and show an increased incidence of skin cancer.
Long Name
Xeroderma Pigmentosum Variant Type Protein
Alternate Names
DNA Polymerase, eta, POLH, RAD30A
Gene Symbol
POLH
UniProt
Additional XPV Products
Product Documents for Human XPV Antibody
Product Specific Notices for Human XPV Antibody
For research use only
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