Human ZBTB38 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5784

R&D Systems, part of Bio-Techne
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AF5784
AF5784-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all ZBTB38 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ZBTB38
Met1-Tyr232
Accession # Q8NAP3

Specificity

Detects endogenous human ZBTB38 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human ZBTB38 Antibody

Detection of Human ZBTB38 antibody by Western Blot.

Detection of Human ZBTB38 by Western Blot.

Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF membrane was probed with 1 µg/mL of Human ZBTB38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5784) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ZBTB38 at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human ZBTB38 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: RPMI 8226 human multiple myeloma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZBTB38

ZBTB38 (Zinc finger and BTB domain-containing protein 38; also CIBZ and Zenon) is an early member of a family of Kaiso-like DNA binding proteins. Although its predicted MW is 134 kDa, it runs anomalously in SDS-PAGE at 175 kDa. ZBTB38 binds to singly methylated CpG (cytosine-phosphate-guanine) motifs. It is considered a transcriptional repressor that either homodimerizes, or heterodimerizes with ZBTB4, and subsequently interacts with either CtBP-1 or -2 to mediate repression. Human ZBTB38 is 1195 amino acids (aa) in length. It contains a BTB domain (aa 33‑100), an RD2 region (aa 158‑341) and ten C2H2-type zinc finger domains (aa 342‑1147). There is one potential alternate start site at Met595. Over aa 1‑232, human ZBTB38 shares 96% aa identity with mouse ZBTB38.

Long Name

Zinc Finger and BTB Domain-Containing Protein 38

Alternate Names

CIBZ, STQTL10

Entrez Gene IDs

253461 (Human); 245007 (Mouse); 315936 (Rat)

Gene Symbol

ZBTB38

UniProt

Q8NAP3

Additional ZBTB38 Products

Product Documents for Human ZBTB38 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ZBTB38 Antibody

For research use only

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