HuR/ELAVL1 Antibody (9Q6L3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15820

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15820-100ul
NBP3-15820-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9Q6L3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HuR/ELAVL1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR/ELAVL1 (Q15717). MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HuR/ELAVL1 Antibody (9Q6L3)

Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]

Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]

Western Blot: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] - Western blot analysis of extracts of various cell lines, using HuR/ELAVL1 antibody (NBP3-15820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]

Immunocytochemistry/ Immunofluorescence: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]

Immunocytochemistry/Immunofluorescence: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] - Immunofluorescence analysis of U-2 OS cells using HuR/ELAVL1 antibody (NBP3-15820). Blue: DAPI for nuclear staining.
Knockout Validated: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]

Knockout Validated: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820]

Knockout Validated: HuR/ELAVL1 Antibody (9Q6L3) [NBP3-15820] - Western blot analysis of extracts from normal (control) and HuR/ELAVL1 knockout (KO) 293T cells, using HuR/ELAVL1 antibody (NBP3-15820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
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Applications for HuR/ELAVL1 Antibody (9Q6L3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HuR/ELAVL1

HuR / ELAVL1 is encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression.

Alternate Names

ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R), ELAV-like protein 1, embryonic lethal, abnormal vision, drosophila, homolog-like 1, Hu antigen R, Hua, Hu-antigen R, HuR, HURELAV1, MelG

Gene Symbol

ELAVL1

Additional HuR/ELAVL1 Products

Product Documents for HuR/ELAVL1 Antibody (9Q6L3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HuR/ELAVL1 Antibody (9Q6L3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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