HUS1B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77749
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for HUS1B Antibody
Immunogen
This affinity purified HUS1B Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 100-125 of human HUS1B protein. (Uniprot: Q8NHY5)
Reactivity Notes
A BLAST analysis indicates 100% homology of the immunizing sequence with HUS1B protein from human. Partial reactivity with mouse HUS1B is not likely based on low (~64%, 9/14) sequence homology. Reactivity against homologues from other sources is not known.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This affinity-purified antibody is directed against human Hus1B protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for HUS1B Antibody
![Western Blot: HUS1B Antibody [NBP1-77749]](https://resources.bio-techne.com/images/products/HUS1B-Antibody-Western-Blot-NBP1-77749-img0002.jpg "Western Blot: HUS1B Antibody [NBP1-77749]")
Western Blot: HUS1B Antibody [NBP1-77749]
Western Blot: HUS1B Antibody [NBP1-77749] - Shows detection of a 36kDa band corresponding to Hus1B in a HeLa cell lysate (arrowhead). The staining pattern in 293 cells is less clear, showing a predominant band at 39 kDa. Personal Communication, A-Lien Lu-Chang, U. Maryland, Baltimore, MDApplications for HUS1B Antibody
Application
Recommended Usage
ELISA
1:20000-1:100000
Immunoprecipitation
1:100
Western Blot
1:500-1:5000
Application Notes
This product has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size corresponding to Hus1B protein by western blotting in the appropriate cell lysate or extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: HUS1B
Alternate Names
checkpoint protein HUS1B, hHUS1B, HUS1 (S. pombe) checkpoint homolog b, HUS1 checkpoint homolog b (S. pombe), MGC126746, MGC126748, RP11-532F6.1
Gene Symbol
HUS1B
UniProt
Additional HUS1B Products
Product Documents for HUS1B Antibody
Product Specific Notices for HUS1B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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