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HUWE1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83127

Novus Biologicals, part of Bio-Techne
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NBP1-83127
NBP1-83127-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLE

Reactivity Notes

Rat (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HUWE1 Antibody

Immunocytochemistry/ Immunofluorescence: HUWE1 Antibody [NBP1-83127]

Immunocytochemistry/ Immunofluorescence: HUWE1 Antibody [NBP1-83127]

Immunocytochemistry/Immunofluorescence: HUWE1 Antibody [NBP1-83127] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: HUWE1 Antibody [NBP1-83127]

Immunohistochemistry-Paraffin: HUWE1 Antibody [NBP1-83127]

Immunohistochemistry-Paraffin: HUWE1 Antibody [NBP1-83127] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells of seminiferus ducts.

Applications for HUWE1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HUWE1

ARFBP1 is a novel E3 ubiquitin ligase. It interacts with p19ARF and its ligase activity is inhibited by this interaction.

Alternate Names

arf-bp1, EC 6.3.2, EC 6.3.2.6, HECT, UBA and WWE domain containing 1, HECTH9, HSPC272, KIAA0312MULE, Mcl-1 ubiquitin ligase E3, Mule, UREB1UBA and WWE domain-containing protein 1, URE-binding protein 1

Gene Symbol

HUWE1

Additional HUWE1 Products

Product Documents for HUWE1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HUWE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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