HUWE1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83127
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (99%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for HUWE1 Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SGSSTTRLTQGIGRSQRTLRQLTANTGHTIHVHYPGNRQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAGTLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLE
Reactivity Notes
Rat (86%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HUWE1 Antibody
![Immunocytochemistry/ Immunofluorescence: HUWE1 Antibody [NBP1-83127]](https://resources.bio-techne.com/images/products/HUWE1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83127-img0004.jpg "Immunocytochemistry/ Immunofluorescence: HUWE1 Antibody [NBP1-83127]")
Immunocytochemistry/ Immunofluorescence: HUWE1 Antibody [NBP1-83127]
Immunocytochemistry/Immunofluorescence: HUWE1 Antibody [NBP1-83127] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.![Immunohistochemistry-Paraffin: HUWE1 Antibody [NBP1-83127]](https://resources.bio-techne.com/images/products/HUWE1-Antibody-Immunohistochemistry-Paraffin-NBP1-83127-img0002.jpg "Immunohistochemistry-Paraffin: HUWE1 Antibody [NBP1-83127]")
Immunohistochemistry-Paraffin: HUWE1 Antibody [NBP1-83127]
Immunohistochemistry-Paraffin: HUWE1 Antibody [NBP1-83127] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells of seminiferus ducts.Applications for HUWE1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HUWE1
Alternate Names
arf-bp1, EC 6.3.2, EC 6.3.2.6, HECT, UBA and WWE domain containing 1, HECTH9, HSPC272, KIAA0312MULE, Mcl-1 ubiquitin ligase E3, Mule, UREB1UBA and WWE domain-containing protein 1, URE-binding protein 1
Gene Symbol
HUWE1
Additional HUWE1 Products
Product Documents for HUWE1 Antibody
Product Specific Notices for HUWE1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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