Hyaluronan synthase 1 Antibody (5B5B4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27181

Novus Biologicals, part of Bio-Techne
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NBP3-27181-0.025ml
NBP3-27181-0.1ml
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 5B5B4

Concentration

1 mg/ml

View all Hyaluronan synthase 1 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Hyaluronan synthase 1 (AA: 74-399) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hyaluronan synthase 1 Antibody (5B5B4)

Immunocytochemistry/Immunofluorescence: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] -

Immunocytochemistry/Immunofluorescence: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] - Analysis of Hela cells using Hyaluronan synthase 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western Blot: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] -

Western Blot: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] - Analysis using Hyaluronan synthase 1 mAb against human Hyaluronan synthase 1 (AA: 74-399) recombinant protein. (Expected MW is 40.2 kDa)

Flow Cytometry: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] -

Flow Cytometry: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] - Analysis of SK-OV-3 cells using Hyaluronan synthase 1 mouse mAb (green) and negative control (red).
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Applications for Hyaluronan synthase 1 Antibody (5B5B4)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hyaluronan synthase 1

Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide varietyof organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternatingglucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA issynthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extrudedthrough pore-like structures into the extracellular space. It serves a variety of functions, including space filling,lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during woundhealing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serumconcentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. Inaddition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes,and overexpression of HA receptors has been correlated with tumor metastasis. HAS1 is a member of the newly identifiedvertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homologyto the hasA gene product of Streptococcus pyogenes, a glycosaminoglycan synthetase (DG42) from Xenopus laevis, and arecently described murine hyaluronan synthase. (provided by RefSeq)

Alternate Names

HA synthase 1, HAS, huHAS1, hyaluronan synthase 1, hyaluronate synthase 1, hyaluronic acid synthase 1

Gene Symbol

HAS1

Additional Hyaluronan synthase 1 Products

Product Documents for Hyaluronan synthase 1 Antibody (5B5B4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Hyaluronan synthase 1 Antibody (5B5B4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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