HYLS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12710

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HYLS1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human HYLS1 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for HYLS1 Antibody

Western Blot: HYLS1 Antibody [NBP3-12710]

Western Blot: HYLS1 Antibody [NBP3-12710]

Western Blot: HYLS1 Antibody [NBP3-12710] - Lane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.)
Immunohistochemistry-Paraffin: HYLS1 Antibody [NBP3-12710]

Immunohistochemistry-Paraffin: HYLS1 Antibody [NBP3-12710]

Immunohistochemistry-Paraffin: HYLS1 Antibody [NBP3-12710] - Staining of human HYLS1 in human kidney with rabbit polyclonal antibody at 1:100 dilution.
Immunoprecipitation: HYLS1 Antibody [NBP3-12710]

Immunoprecipitation: HYLS1 Antibody [NBP3-12710]

Immunoprecipitation: HYLS1 Antibody [NBP3-12710] - Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-HYLS1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HYLS1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDa

Applications for HYLS1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HYLS1

HYLS1 encodes a protein localized to the cytoplasm. Mutations in this gene are associated with hydrolethalus syndrome. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Alternate Names

FLJ32915HLS, hydrolethalus syndrome 1, hydrolethalus syndrome protein 1

Gene Symbol

HYLS1

Additional HYLS1 Products

Product Documents for HYLS1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HYLS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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