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HYPE Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-58855

Novus Biologicals, part of Bio-Techne
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NBP2-58855

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: VKKVMSIPKGNSALRRVMEETYYHHIYHTVAIEGNTLTLSEIRHILETRYAVPGKSLEEQNEVIGMHAAMKYINTTLVSRIGSVTISD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HYPE Antibody

Western Blot: HYPE Antibody [NBP2-58855]

Western Blot: HYPE Antibody [NBP2-58855]

Western Blot: HYPE Antibody [NBP2-58855] - Western blot analysis in human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: HYPE Antibody [NBP2-58855]

Immunocytochemistry/ Immunofluorescence: HYPE Antibody [NBP2-58855]

Immunocytochemistry/Immunofluorescence: HYPE Antibody [NBP2-58855] - Staining of human cell line BJ shows localization to nucleus.

Applications for HYPE Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HYPE

Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. Able to inactivate Rho GTPases in vitro by adding AMP to RhoA, Rac and Cdc42. It is however unclear whether it inactivates GTPases in vivo and physiological substrates probably remain to be identified

Alternate Names

AMPylator FICD, EC 2.7.7.n1, FIC domain containing, FIC domain-containing protein, fic S-phase protein cell division homolog, HIP-13, HIP13UNQ3041, huntingtin interacting protein 13, Huntingtin interacting protein E, huntingtin interactor protein E, Huntingtin yeast partner E, Huntingtin-interacting protein 13, Huntingtin-interacting protein E, HYPEadenosine monophosphate-protein transferase FICD, MGC5623

Gene Symbol

FICD

Additional HYPE Products

Product Documents for HYPE Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HYPE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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