IARS2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84638
![Western Blot: IARS2 Antibody [NBP1-84638]](https://resources.bio-techne.com/images/products/IARS2-Antibody-Western-Blot-NBP1-84638-img0010.jpg "Western Blot: IARS2 Antibody [NBP1-84638]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VASQHNLPMDCLVDEDGVFTDVAGPELQNKAVLEEGTDVVIKMLQTAKNLLKEEKLVHSYPYDWRTKKPVVIRASKQWFINITDIKTA
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (81%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IARS2 Antibody
![Immunocytochemistry/ Immunofluorescence: IARS2 Antibody [NBP1-84638]](https://resources.bio-techne.com/images/products/IARS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84638-img0007.jpg "Immunocytochemistry/ Immunofluorescence: IARS2 Antibody [NBP1-84638]")
Immunocytochemistry/ Immunofluorescence: IARS2 Antibody [NBP1-84638]
Immunocytochemistry/Immunofluorescence: IARS2 Antibody [NBP1-84638] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin- IARS2 Antibody [NBP1-84638] -
Immunohistochemistry-Paraffin- IARS2 Antibody [NBP1-84638] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Applications for IARS2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IARS2
Alternate Names
EC 6.1.1, EC 6.1.1.5, FLJ10326, IleRS, isoleucine tRNA ligase 2, mitochondrial, Isoleucine--tRNA ligase, isoleucine-tRNA synthetase 2, mitochondrial, isoleucyl-tRNA synthetase 2, mitochondrial, isoleucyl-tRNA synthetase, mitochondrial, mitochondrial isoleucine tRNA synthetase
Gene Symbol
IARS2
Additional IARS2 Products
Product Documents for IARS2 Antibody
Product Specific Notices for IARS2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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