iASPP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-88155
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human inhibitor of apoptosis stimulating protein of p53 using the numbering given in entry NP_006654.2 (GeneID 10848).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for iASPP Antibody
![Western Blot: iASPP Antibody [NB100-88155]](https://resources.bio-techne.com/images/products/iASPP-Antibody-Western-Blot-NB100-88155-img0005.jpg "Western Blot: iASPP Antibody [NB100-88155]")
Western Blot: iASPP Antibody [NB100-88155]
Western Blot: iASPP Antibody [NB100-88155] - Detection of Human and Mouse iASPP by Western Blot. Samples: Whole cell lysate from 293T (50, 15, 5 ug), HeLa (50, 15 ug), and mouse NIH3T3 (50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-iASPP antibody NB100-88155 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: iASPP Antibody [NB100-88155]](https://resources.bio-techne.com/images/products/iASPP-Antibody-Immunoprecipitation-NB100-88155-img0004.jpg "Immunoprecipitation: iASPP Antibody [NB100-88155]")
Immunoprecipitation: iASPP Antibody [NB100-88155]
Immunoprecipitation: iASPP Antibody [NB100-88155] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antiiASPP antibody used for IP at 6 ug per reaction. iASPP was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated iASPP, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutesApplications for iASPP Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: iASPP
Alternate Names
IASPPinhibitor of apoptosis stimulating protein of p53, Inhibitor of ASPP protein, NFkB-interacting protein 1, NKIP1, PPP1R13BL, PPP1R13B-like protein, Protein iASPP, protein phosphatase 1, regulatory (inhibitor) subunit 13 like, protein phosphatase 1, regulatory subunit 13 like, RAINFkB interacting protein 1, relA-associated inhibitor
Entrez Gene IDs
10848 (Human)
Gene Symbol
PPP1R13L
UniProt
Additional iASPP Products
Product Documents for iASPP Antibody
Product Specific Notices for iASPP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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