IBRDC2 Antibody (OTI9H10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02128
![Flow Cytometry: IBRDC2 Antibody (OTI9H10) [NBP2-02128]](https://resources.bio-techne.com/images/products/IBRDC2-Antibody-9H10-Flow-Cytometry-NBP2-02128-img0002.jpg "Flow Cytometry: IBRDC2 Antibody (OTI9H10) [NBP2-02128]")
![Western Blot: IBRDC2 Antibody (OTI9H10) [NBP2-02128]](https://resources.bio-techne.com/images/products/IBRDC2-Antibody-9H10-Western-Blot-NBP2-02128-img0003.jpg "Western Blot: IBRDC2 Antibody (OTI9H10) [NBP2-02128]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI9H10
Concentration
0.8 mg/ml
Product Summary for IBRDC2 Antibody (OTI9H10)
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-256 of human RNF144B (NP_877434) produced in E. coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
33.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IBRDC2 Antibody (OTI9H10)
Western Blot: IBRDC2 Antibody (OTI9H10) [NBP2-02128]
Western Blot: IBRDC2 Antibody (9H10) [NBP2-02128] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IBRDC2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IBRDC2.
Immunocytochemistry/Immunofluorescence: IBRDC2 Antibody (9H10) [NBP2-02128] Staining of COS7 cells transiently transfected by pCMV6-ENTRY IBRDC2.
![Immunohistochemistry-Paraffin: IBRDC2 Antibody (OTI9H10) [NBP2-02128]](https://resources.bio-techne.com/images/products/IBRDC2-Antibody-9H10-Immunohistochemistry-Paraffin-NBP2-02128-img0009.jpg "Immunohistochemistry-Paraffin: IBRDC2 Antibody (OTI9H10) [NBP2-02128]")
Immunohistochemistry-Paraffin: IBRDC2 Antibody (OTI9H10) [NBP2-02128]
Immunohistochemistry-Paraffin: IBRDC2 Antibody (9H10) [NBP2-02128] - Staining of paraffin-embedded Human prostate tissue using anti-IBRDC2 mouse monoclonal antibody.![Immunocytochemistry/ Immunofluorescence: IBRDC2 Antibody (OTI9H10) [NBP2-02128]](https://resources.bio-techne.com/images/products/IBRDC2-Antibody-9H10-Immunocytochemistry-Immunofluorescence-NBP2-02128-img0004.jpg "Immunocytochemistry/ Immunofluorescence: IBRDC2 Antibody (OTI9H10) [NBP2-02128]")
![Immunohistochemistry-Paraffin: IBRDC2 Antibody (OTI9H10) [NBP2-02128]](https://resources.bio-techne.com/images/products/IBRDC2-Antibody-9H10-Immunohistochemistry-Paraffin-NBP2-02128-img0005.jpg "Immunohistochemistry-Paraffin: IBRDC2 Antibody (OTI9H10) [NBP2-02128]")
![Immunohistochemistry-Paraffin: IBRDC2 Antibody (OTI9H10) [NBP2-02128]](https://resources.bio-techne.com/images/products/IBRDC2-Antibody-9H10-Immunohistochemistry-Paraffin-NBP2-02128-img0006.jpg "Immunohistochemistry-Paraffin: IBRDC2 Antibody (OTI9H10) [NBP2-02128]")
![Immunohistochemistry-Paraffin: IBRDC2 Antibody (OTI9H10) [NBP2-02128]](https://resources.bio-techne.com/images/products/IBRDC2-Antibody-9H10-Immunohistochemistry-Paraffin-NBP2-02128-img0007.jpg "Immunohistochemistry-Paraffin: IBRDC2 Antibody (OTI9H10) [NBP2-02128]")
![Immunohistochemistry-Paraffin: IBRDC2 Antibody (OTI9H10) [NBP2-02128]](https://resources.bio-techne.com/images/products/IBRDC2-Antibody-9H10-Immunohistochemistry-Paraffin-NBP2-02128-img0008.jpg "Immunohistochemistry-Paraffin: IBRDC2 Antibody (OTI9H10) [NBP2-02128]")
![Flow Cytometry: IBRDC2 Antibody (OTI9H10) [NBP2-02128]](https://resources.bio-techne.com/images/products/IBRDC2-Antibody-9H10-Flow-Cytometry-NBP2-02128-img0001.jpg "Flow Cytometry: IBRDC2 Antibody (OTI9H10) [NBP2-02128]")
Applications for IBRDC2 Antibody (OTI9H10)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:50-100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.8 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IBRDC2
Alternate Names
bA528A10.3, E3 ubiquitin-protein ligase RNF144B, EC 6.3.2, EC 6.3.2.-, IBR domain containing 2, IBR domain-containing protein 2, IBRDC2, KIAA0161, p53-inducible RING finger protein, p53RFP, PIR2, ring finger protein 144BMGC71786
Entrez Gene IDs
255488 (Human)
Gene Symbol
RNF144B
Additional IBRDC2 Products
Product Documents for IBRDC2 Antibody (OTI9H10)
Product Specific Notices for IBRDC2 Antibody (OTI9H10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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