IBTK Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50033
![Western Blot: IBTK Antibody [NBP1-50033]](https://resources.bio-techne.com/images/products/IBTK-Antibody-Western-Blot-NBP1-50033-img0004.jpg "Western Blot: IBTK Antibody [NBP1-50033]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 1050 and 1100 of human Inhibitor of Bruton Agammaglobulinemia Tyrosine Kinase, using the numbering given in entry NP_056340.2 (GeneID 25998).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IBTK Antibody
Western Blot: IBTK Antibody [NBP1-50033]
Western Blot: IBTK Antibody [NBP1-50033] - Detection of Human IBtk by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-IBtk antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IBtk was also immunoprecipitated by rabbit anti-IBtk antibody BL10880, which recognizes an upstream epitope. For blotting immunoprecipitated IBtk, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).Applications for IBTK Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 mcg/mg
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: IBTK
Alternate Names
Bruton agammaglobulinemia tyrosine kinase inhibitor, BTK-binding protein, BTKI, DKFZP564B116, IBtk, inhibitor of Bruton agammaglobulinemia tyrosine kinase, inhibitor of Bruton tyrosine kinase, inhibitor of Bruton's tyrosine kinase, inhibitor of Bruton's tyrosine kinase-alpha, inhibitor of Bruton's tyrosine kinase-beta, inhibitor of Bruton's tyrosine kinase-gamma, KIAA1417, MGC142256, MGC142258, RP1-93K22.1
Gene Symbol
IBTK
Additional IBTK Products
Product Documents for IBTK Antibody
Product Specific Notices for IBTK Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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