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IBTK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88512

Novus Biologicals, part of Bio-Techne
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NBP1-88512
NBP1-88512-25ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LKEICEVALTEKLTLKNAAMLLEFAAMYSAKQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSEFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDIFLKEEINMEQNHSETMFKKAK

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30975981).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IBTK Antibody

Immunocytochemistry/ Immunofluorescence: IBTK Antibody [NBP1-88512]

Immunocytochemistry/ Immunofluorescence: IBTK Antibody [NBP1-88512]

Immunocytochemistry/Immunofluorescence: IBTK Antibody [NBP1-88512] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: IBTK Antibody [NBP1-88512]

Immunohistochemistry-Paraffin: IBTK Antibody [NBP1-88512]

Immunohistochemistry-Paraffin: IBTK Antibody [NBP1-88512] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Simple Western: IBTK Antibody [NBP1-88512]

Simple Western: IBTK Antibody [NBP1-88512]

Simple Western: IBTK Antibody [NBP1-88512] - Simple Western lane view shows a specific band for IBTK in 0.2 mg/ml of U-251 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for IBTK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:20

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Immunoprecipitation and western blot reported in scientific literature (PMID: 28710282). ICC/IF reported in scientific literature (PMID: 29317636).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IBTK

IBTK is encoded by this gene binds to Bruton's tyrosine kinase (BTK) and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. [provided by RefSeq]

Alternate Names

Bruton agammaglobulinemia tyrosine kinase inhibitor, BTK-binding protein, BTKI, DKFZP564B116, IBtk, inhibitor of Bruton agammaglobulinemia tyrosine kinase, inhibitor of Bruton tyrosine kinase, inhibitor of Bruton's tyrosine kinase, inhibitor of Bruton's tyrosine kinase-alpha, inhibitor of Bruton's tyrosine kinase-beta, inhibitor of Bruton's tyrosine kinase-gamma, KIAA1417, MGC142256, MGC142258, RP1-93K22.1

Gene Symbol

IBTK

Additional IBTK Products

Product Documents for IBTK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IBTK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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