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ICA1 Antibody (6G11)

Novus Biologicals, part of Bio-Techne | Catalog # H00003382-M01

Novus Biologicals, part of Bio-Techne
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H00003382-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6G11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ICA1 (NP_071682, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGHKCSYPWDLQDRYAQDKSVVNKMQQKYWETKQAFIKATGKKEDEHVVASDADLDAKLELFHSIQRTCLDLSKAIVLYQKRICFLSQEENELGKFLRSQGFQDKTRAG

Localization

Cell Membrane, Cytoplasmic and Golgi Apparatus

Specificity

ICA1 - islet cell autoantigen 1, 69kDa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ICA1 Antibody (6G11)

Western Blot: ICA1 Antibody (6G11) [H00003382-M01]

Western Blot: ICA1 Antibody (6G11) [H00003382-M01]

Western Blot: ICA1 Antibody (6G11) [H00003382-M01] - Analysis of ICA1 expression in transfected 293T cell line by ICA1 monoclonal antibody (M01), clone 6G11.Lane 1: ICA1 transfected lysate (Predicted MW: 54.6 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: ICA1 Antibody (6G11) [H00003382-M01] - Detection limit for recombinant GST tagged ICA1 is approximately 0.3ng/ml as a capture antibody.

Applications for ICA1 Antibody (6G11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ICA1

This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Long Name

Islet Cell Autoantigen 1

Alternate Names

ICAp69

Entrez Gene IDs

3382 (Human)

Gene Symbol

ICA1

OMIM

147625 (Human)

Additional ICA1 Products

Product Documents for ICA1 Antibody (6G11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ICA1 Antibody (6G11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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