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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # ICAM 3.2

Format

BSA Free

Concentration

1 mg/ml

Product Summary for ICAM-3/CD50 Antibody (ICAM 3.2) - BSA Free

Immunogen

ICAM-3/Fc chimeric fusion protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for ICAM-3/CD50 Antibody (ICAM 3.2) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Radioimmunoassay

1:100 - 1:2000

Western Blot

1:100 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ICAM-3/CD50

All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM 3 is the major ligand for LFA-1 in the resting state.

Long Name

Intercellular Adhesion Molecule 3

Alternate Names

CD50, ICAM3

Gene Symbol

ICAM3

Additional ICAM-3/CD50 Products

Product Documents for ICAM-3/CD50 Antibody (ICAM 3.2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ICAM-3/CD50 Antibody (ICAM 3.2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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