Key Product Details

Species Reactivity

Human, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E7

Concentration

1 mg/ml

View all ICAM-3/CD50 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ICAM3(NP_002153) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ICAM-3/CD50 Antibody (OTI1E7)

Western Blot: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010]

Western Blot: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010]

Western Blot: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM-3.
Immunohistochemistry: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010]

Immunohistochemistry: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010]

Immunohistochemistry: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010]

Western Blot: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010]

Western Blot: ICAM-3/CD50 Antibody (OTI1E7) [NBP2-46010] - Analysis of extracts (35ug) from 9 different cell lines by using ICAM3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Applications for ICAM-3/CD50 Antibody (OTI1E7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:400-4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ICAM-3/CD50

All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM 3 is the major ligand for LFA-1 in the resting state.

Long Name

Intercellular Adhesion Molecule 3

Alternate Names

CD50, ICAM3

Gene Symbol

ICAM3

Additional ICAM-3/CD50 Products

Product Documents for ICAM-3/CD50 Antibody (OTI1E7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ICAM-3/CD50 Antibody (OTI1E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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