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ICAM-3/CD50 Antibody (SR1003)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22536

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-22536-100ul
NBP3-22536-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1003

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for ICAM-3/CD50 Antibody (SR1003)

Immunogen

A synthesized peptide derived from human ICAM-3/CD50 (Uniprot #: P32942)

Specificity

Detects endogenous levels of total ICAM-3/CD50

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ICAM-3/CD50 Antibody (SR1003)

Western Blot: ICAM-3/CD50 Antibody (SR1003) [NBP3-22536] -

Western Blot: ICAM-3/CD50 Antibody (SR1003) [NBP3-22536] - Western blot analysis using NBP3-22536 in Ramos cell lysate.
ICAM-3/CD50 Antibody (SR1003)

Western Blot: ICAM-3/CD50 Antibody (SR1003) [NBP3-22536] -

Western Blot: ICAM-3/CD50 Antibody (SR1003) [NBP3-22536] - Western blot analysis of ICAM-3/CD50 expression in Ramos cell lysate.

Applications for ICAM-3/CD50 Antibody (SR1003)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ICAM-3/CD50

All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM 3 is the major ligand for LFA-1 in the resting state.

Long Name

Intercellular Adhesion Molecule 3

Alternate Names

CD50, ICAM3

Gene Symbol

ICAM3

Additional ICAM-3/CD50 Products

Product Documents for ICAM-3/CD50 Antibody (SR1003)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ICAM-3/CD50 Antibody (SR1003)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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