ID1 Antibody (CL11995)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15127
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL11995
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for ID1 Antibody (CL11995)
Immunogen
This antibody was developed against a synthetic peptide. Actual immunogen sequence is proprietary information.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for ID1 Antibody (CL11995)
Immunocytochemistry/Immunofluorescence: ID1 Antibody-
Immunocytochemistry/Immunofluorescence: ID1 Antibody (CL11995) [NBP3-15127] - Staining of PC-3 cells using the Anti-ID1 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probe is visualized in red.
Immunohistochemistry-Paraffin: ID1 Antibody-
Immunohistochemistry-Paraffin: ID1 Antibody (CL11995) [NBP3-15127] - Staining of human liver shows weak nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: ID1 Antibody (CL11995) [NBP3-15127] -
Immunohistochemistry-Paraffin: ID1 Antibody (CL11995) [NBP3-15127] - Staining of human colon shows strong positivity in glandular cells.
Applications for ID1 Antibody (CL11995)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2), 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ID1
Long Name
Inhibitor of DNA Binding 1
Alternate Names
BHLHB24, bHLHb24DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, dJ857M17.1.2, dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helixprotein), ID, inhibitor of differentiation 1, Inhibitor of DNA binding 1, inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
Gene Symbol
ID1
Additional ID1 Products
Product Documents for ID1 Antibody (CL11995)
Product Specific Notices for ID1 Antibody (CL11995)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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