ID1 Antibody (PCRP-ID1-2F11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13940
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # PCRP-ID1-2F11
Concentration
0.2 mg/ml
Product Summary for ID1 Antibody (PCRP-ID1-2F11)
Immunogen
Recombinant fragment (aa 5-150) of human ID1 (Uniprot: P41134)
Localization
Nucleus, Cytoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
16.13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14075)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for ID1 Antibody (PCRP-ID1-2F11)
Immunocytochemistry/ Immunofluorescence: ID1 Antibody (PCRP-ID1-2F11) [NBP3-13940]
Immunocytochemistry/Immunofluorescence: ID1 Antibody (PCRP-ID1-2F11) [NBP3-13940] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using ID1 Antibody (PCRP-ID1-2F11). followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).Flow Cytometry: ID1 Antibody (PCRP-ID1-2F11) [NBP3-13940]
Flow Cytometry: ID1 Antibody (PCRP-ID1-2F11) [NBP3-13940] - Flow cytometric analysis of PFA-fixed HeLa cells. ID1 antibody (PCRP-ID1-2F11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).Protein Array: ID1 Antibody (PCRP-ID1-2F11) [NBP3-13940]
Protein Array: ID1 Antibody (PCRP-ID1-2F11) [NBP3-13940] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ID1 Antibody (PCRP-ID1-2F11).Applications for ID1 Antibody (PCRP-ID1-2F11)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunoprecipitation
1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: ID1
Long Name
Inhibitor of DNA Binding 1
Alternate Names
BHLHB24, bHLHb24DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, dJ857M17.1.2, dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helixprotein), ID, inhibitor of differentiation 1, Inhibitor of DNA binding 1, inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
Gene Symbol
ID1
UniProt
Additional ID1 Products
Product Documents for ID1 Antibody (PCRP-ID1-2F11)
Product Specific Notices for ID1 Antibody (PCRP-ID1-2F11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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