ID2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27194

Novus Biologicals, part of Bio-Techne
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NBP2-27194
NBP2-27194SS
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Bovine, Chicken, Equine, Hamster, Primate

Predicted:

Chimpanzee (100%), Monkey (100%), Mouse (100%), Orangutan (100%), Porcine (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ID2 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 60-110 of human ID2 was used as the immunogen for this antibody.

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in White-cheeked Gibbon, Macaque, Golden Hamster, 89% in New World Monkey, Chicken, and 83% in Opossum.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ID2 Antibody

Western Blot: ID2 Antibody [NBP2-27194]

Western Blot: ID2 Antibody [NBP2-27194]

Western Blot: ID2 Antibody [NBP2-27194] - Analysis using ID2 antibody. Lysate from human brain in the 1) absence and 2) presence of immunizing peptide, 3) mouse and 4) rat brain probed with ID2 antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunocytochemistry/ Immunofluorescence: ID2 Antibody [NBP2-27194]

Immunocytochemistry/ Immunofluorescence: ID2 Antibody [NBP2-27194]

Immunocytochemistry/Immunofluorescence: ID2 Antibody [NBP2-27194] - IF Confocal analysis of A549 cells using ID2 antibody (NBP2-27194, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue). Image from verified customer review.

Applications for ID2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported by customer review

Western Blot

1-2ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-27194 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ID2

ID2 protein belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA bindingThis protein may play a role in negatively regulating cell differentiation. ID2 may be an inhibitor of tissue-specific gene expression. Expression of ID2 is high in early fetal tissues, including those of the central nervous system.

Long Name

Inhibitor of DNA Binding 2

Alternate Names

GIG8

Gene Symbol

ID2

Additional ID2 Products

Product Documents for ID2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ID2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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