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ID3 Antibody (2H8)

Novus Biologicals, part of Bio-Techne | Catalog # H00003399-M03

Novus Biologicals, part of Bio-Techne
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H00003399-M03

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2H8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ID3 (NP_002158, 1 a.a. ~ 83 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKALSPVRGCYEAVCCLSERSLAIARGRGKGPAAEEPLSLLDDMNHCYSRLRELVPGVPRGTQLSQVEILQRVIDYILDLQVV

Specificity

ID3 (2H8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ID3 Antibody (2H8)

Western Blot: ID3 Antibody (2H8) [H00003399-M03]

Western Blot: ID3 Antibody (2H8) [H00003399-M03]

Western Blot: ID3 Antibody (2H8) [H00003399-M03] - Analysis of ID3 expression in HeLa (Cat # L013V1).
Immunocytochemistry/ Immunofluorescence: ID3 Antibody (2H8) [H00003399-M03]

Immunocytochemistry/ Immunofluorescence: ID3 Antibody (2H8) [H00003399-M03]

Immunocytochemistry/Immunofluorescence: ID3 Antibody (2H8) [H00003399-M03] - Analysis of monoclonal antibody to ID3 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: ID3 Antibody (2H8) [H00003399-M03]

ELISA: ID3 Antibody (2H8) [H00003399-M03]

ELISA: ID3 Antibody (2H8) [H00003399-M03] - Detection limit for recombinant GST tagged ID3 is approximately 1ng/ml as a capture antibody.

Applications for ID3 Antibody (2H8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ID3

Members of the ID family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA.[supplied by OMIM]

Alternate Names

BHLHB25, bHLHb251R21, Class B basic helix-loop-helix protein 25, DNA-binding protein inhibitor ID-3, HEIR1, HEIR-1, Helix-loop-helix protein HEIR-1, ID-like protein inhibitor HLH 1R21, Inhibitor of DNA binding 3, inhibitor of DNA binding 3, dominant negative helix-loop-helix protein

Entrez Gene IDs

3399 (Human)

Gene Symbol

ID3

OMIM

600277 (Human)

Additional ID3 Products

Product Documents for ID3 Antibody (2H8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ID3 Antibody (2H8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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