ID3 Antibody (4G1)
Novus Biologicals, part of Bio-Techne | Catalog # H00003399-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4G1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ID3 (NP_002158, 1 a.a. ~ 83 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKALSPVRGCYEAVCCLSERSLAIARGRGKGPAAEEPLSLLDDMNHCYSRLRELVPGVPRGTQLSQVEILQRVIDYILDLQVV
Specificity
ID3 - inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for ID3 Antibody (4G1)
![Western Blot: ID3 Antibody (4G1) [H00003399-M01]](https://resources.bio-techne.com/images/products/ID3-Antibody-4G1-Western-Blot-H00003399-M01-img0006.jpg "Western Blot: ID3 Antibody (4G1) [H00003399-M01]")
Western Blot: ID3 Antibody (4G1) [H00003399-M01]
Western Blot: ID3 Antibody (4G1) [H00003399-M01] - ID3 monoclonal antibody (M01), clone 4G1 Analysis of ID3 expression in HeLa.![Western Blot: ID3 Antibody (4G1) [H00003399-M01]](https://resources.bio-techne.com/images/products/ID3-Antibody-4G1-Western-Blot-H00003399-M01-img0004.jpg "Western Blot: ID3 Antibody (4G1) [H00003399-M01]")
Western Blot: ID3 Antibody (4G1) [H00003399-M01]
Western Blot: ID3 Antibody (4G1) [H00003399-M01] - Analysis of ID3 over-expressed 293 cell line, cotransfected with ID3 Validated Chimera RNAi ( Cat # H00003399-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ID3 monoclonal antibody (M01), clone 4G1 (Cat # H00003399-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: ID3 Antibody (4G1) [H00003399-M01] - Detection limit for recombinant GST tagged ID3 is approximately 0.03ng/ml as a capture antibody.
Applications for ID3 Antibody (4G1)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ID3
Alternate Names
BHLHB25, bHLHb251R21, Class B basic helix-loop-helix protein 25, DNA-binding protein inhibitor ID-3, HEIR1, HEIR-1, Helix-loop-helix protein HEIR-1, ID-like protein inhibitor HLH 1R21, Inhibitor of DNA binding 3, inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
Entrez Gene IDs
3399 (Human)
Gene Symbol
ID3
OMIM
600277 (Human)
UniProt
Additional ID3 Products
Product Documents for ID3 Antibody (4G1)
Product Specific Notices for ID3 Antibody (4G1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...