ID3 Antibody (OTI8B3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70979

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI8B3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ID3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ID3 (NP_002158) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

12.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ID3 Antibody (OTI8B3) - Azide and BSA Free

ID3 Antibody (OTI8B3) - Azide and BSA Free

Western Blot: Mouse ID3 mAb (OTI8B3) - Azide and BSA Free [NBP2-70979]

Western Blot: Mouse ID3 mAb (OTI8B3) - Azide and BSA Free [NBP2-70979] - Analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1), human ID3 plasmid (RC200583, lane 2), mouse ID3 plasmid (MR227292, lane 3) using anti-ID3 antibody at 1:500.
Immunocytochemistry/ Immunofluorescence: ID3 Antibody (OTI8B3) - Azide and BSA Free [NBP2-70979]

Immunocytochemistry/ Immunofluorescence: ID3 Antibody (OTI8B3) - Azide and BSA Free [NBP2-70979]

Immunocytochemistry/Immunofluorescence: ID3 Antibody (OTI8B3) - Azide and BSA Free [NBP2-70979] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ID3.
Immunohistochemistry: ID3 Antibody (OTI8B3) - Azide and BSA Free [NBP2-70979]

Immunohistochemistry: ID3 Antibody (OTI8B3) - Azide and BSA Free [NBP2-70979]

Immunohistochemistry: ID3 Antibody (OTI8B3) - Azide and BSA Free [NBP2-70979] - Staining of paraffin-embedded Human prostate tissue using anti-ID3 mouse monoclonal antibody.
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Applications for ID3 Antibody (OTI8B3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ID3

Members of the ID family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA.[supplied by OMIM]

Alternate Names

BHLHB25, bHLHb251R21, Class B basic helix-loop-helix protein 25, DNA-binding protein inhibitor ID-3, HEIR1, HEIR-1, Helix-loop-helix protein HEIR-1, ID-like protein inhibitor HLH 1R21, Inhibitor of DNA binding 3, inhibitor of DNA binding 3, dominant negative helix-loop-helix protein

Gene Symbol

ID3

Additional ID3 Products

Product Documents for ID3 Antibody (OTI8B3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ID3 Antibody (OTI8B3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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