IDH3G Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78786

Novus Biologicals, part of Bio-Techne
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NBP1-78786
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all IDH3G Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DHLKLHSYATSIRK corresponding to internal region according to NP_777358.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_004126.1; NP_777358.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for IDH3G Antibody

Western Blot: IDH3G Antibody [NBP1-78786]

Western Blot: IDH3G Antibody [NBP1-78786]

Western Blot: IDH3G Antibody [NBP1-78786] - Analysis of Rat (A) and Pig (B) Heart lysate (35ug protein in RIPA buffer).
Western Blot: IDH3G Antibody [NBP1-78786]

Western Blot: IDH3G Antibody [NBP1-78786]

Western Blot: IDH3G Antibody [NBP1-78786] - Analysis of MCF7 lysate (35ug protein in RIPA buffer).

Applications for IDH3G Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx. 38 kDa band observed in lysates of MCF7 and of human, mouse, rat and porcine heart (calculated MW of 41.5 kDa band according to NP_777358.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IDH3G

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined. [provided by RefSeq]

Alternate Names

EC 1.1.1, EC 1.1.1.41, H-IDHG, IDH-gamma, isocitrate dehydrogenase 3 (NAD+) gamma, isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, gamma subunit, mitochondrial, NAD (H)-specific isocitrate dehydrogenase gamma subunit, NAD+-specific ICDH

Entrez Gene IDs

3421 (Human); 15929 (Mouse); 25179 (Rat)

Gene Symbol

IDH3G

UniProt

NP_004126.1 (Human)

Additional IDH3G Products

Product Documents for IDH3G Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IDH3G Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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