IDI2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97025

Novus Biologicals, part of Bio-Techne
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NBP2-97025-100ul
NBP2-97025-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IDI2 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human IDI2 (Uniprot#: Q9BXS1; Met1-Val227)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for IDI2 Antibody

Western Blot: IDI2 Antibody [NBP2-97025]

Western Blot: IDI2 Antibody [NBP2-97025]

Western Blot: IDI2 Antibody [NBP2-97025] - Anti-IDI2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 30 kDa
Immunohistochemistry-Paraffin: IDI2 Antibody [NBP2-97025]

Immunohistochemistry-Paraffin: IDI2 Antibody [NBP2-97025]

Immunohistochemistry-Paraffin: IDI2 Antibody [NBP2-97025] - Immunochemical staining of human IDI2 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: IDI2 Antibody [NBP2-97025]

Immunoprecipitation: IDI2 Antibody [NBP2-97025]

Immunoprecipitation: IDI2 Antibody [NBP2-97025] - IDI2 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 4 ul anti-IDI2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-IDI2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 27 kDa

Applications for IDI2 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

4-8 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IDI2

IDI2 catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated byATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

Alternate Names

diphosphate dimethylallyl diphosphate isomerase 2, EC 5.3.3.2, IPP isomerase 2, IPPI2isopentenyl-diphosphate Delta-isomerase 2, Isopentenyl pyrophosphate isomerase 2, isopentenyl-diphosphate delta isomerase 2

Gene Symbol

IDI2

Additional IDI2 Products

Product Documents for IDI2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IDI2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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