Iduronate 2-Sulfatase/IDS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28477

Novus Biologicals, part of Bio-Techne
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NBP1-28477
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Iduronate 2-Sulfatase/IDS Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KHFRFRDLEEDP corresponding to internal region according to NP_000193.1.

Specificity

This antibody is expected to recognize isoform a (NP_000193.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Iduronate 2-Sulfatase/IDS Antibody

Western Blot: Iduronate 2-Sulfatase/IDS Antibody [NBP1-28477]

Western Blot: Iduronate 2-Sulfatase/IDS Antibody [NBP1-28477]

Western Blot: Iduronate 2-Sulfatase/IDS Antibody [NBP1-28477] - (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody [NBP1-28477]

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody [NBP1-28477]

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody [NBP1-28477] - (3.8ug/ml) staining of Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Iduronate 2-Sulfatase/IDS Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in human liver lysates (calculated MW of 61.9 kDa band according to NP_000193.1). IHC-P: Human placenta shows lysosomal staining of trophoblasts.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Iduronate 2-Sulfatase/IDS

Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome. Iduronate-2-sulfatase has a strong sequence homology with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase. A splice variant of this gene has been described.

Alternate Names

SIDS

Entrez Gene IDs

3423 (Human)

Gene Symbol

IDS

UniProt

NP_000193.1 (Human)

Additional Iduronate 2-Sulfatase/IDS Products

Product Documents for Iduronate 2-Sulfatase/IDS Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Iduronate 2-Sulfatase/IDS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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