IFITM1 Antibody (021)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89230
Recombinant Monoclonal Antibody
![Western Blot: IFITM1 Antibody (021) [NBP2-89230]](https://resources.bio-techne.com/images/products/IFITM1-Antibody-021-Western-Blot-NBP2-89230-img0001.jpg "Western Blot: IFITM1 Antibody (021) [NBP2-89230]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 021
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human IFITM1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for IFITM1 Antibody (021)
Western Blot: IFITM1 Antibody (021) [NBP2-89230]
Western Blot: IFITM1 Antibody (021) [NBP2-89230] - Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDa Observed band size:18 kDa![Immunocytochemistry/ Immunofluorescence: IFITM1 Antibody (021) [NBP2-89230]](https://resources.bio-techne.com/images/products/IFITM1-Antibody-021-Immunocytochemistry-Immunofluorescence-NBP2-89230-img0003.jpg "Immunocytochemistry/ Immunofluorescence: IFITM1 Antibody (021) [NBP2-89230]")
Immunocytochemistry/ Immunofluorescence: IFITM1 Antibody (021) [NBP2-89230]
Immunocytochemistry/Immunofluorescence: IFITM1 Antibody (021) [NBP2-89230] - Staining of IFITM1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human IFITM1 monoclonal antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.![Immunoprecipitation: IFITM1 Antibody (021) [NBP2-89230]](https://resources.bio-techne.com/images/products/IFITM1-Antibody-021-Immunoprecipitation-NBP2-89230-img0002.jpg "Immunoprecipitation: IFITM1 Antibody (021) [NBP2-89230]")
Immunoprecipitation: IFITM1 Antibody (021) [NBP2-89230]
Immunoprecipitation: IFITM1 Antibody (021) [NBP2-89230] - Lane A:0.5 mg K562 Whole Cell Lysate 2 uL anti-CD225 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-CD225 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 15 kDa Observed band size: 15 kDaApplications for IFITM1 Antibody (021)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IFITM1
Long Name
Interferon-induced Transmembrane Protein 1
Alternate Names
CD225, IFI17, Leu-13
Gene Symbol
IFITM1
Additional IFITM1 Products
Product Documents for IFITM1 Antibody (021)
Product Specific Notices for IFITM1 Antibody (021)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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