IFN-alpha/beta R1 Antibody (SR45-08)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67339
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Applications
Validated:
Flow Cytometry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SR45-08
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human IFN-alpha/beta R1 aa 508-557 / 557. (SwissProt: P17181 Human; SwissProt: P33896 Mouse; SwissProt: D3ZDS9 Rat)
Localization
Cell membrane, Late endosome, Lysosome.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IFN-alpha/beta R1 Antibody (SR45-08)
Western Blot: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339]
Western Blot: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Analysis using Siha cell lysates at 1/500 dilution.Immunohistochemistry-Paraffin: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339]
Immunohistochemistry-Paraffin: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IFN-alpha/beta R1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-37, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Flow Cytometry: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339]
Flow Cytometry: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Analysis of Jurkat cells with IFNAR1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Applications for IFN-alpha/beta R1 Antibody (SR45-08)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IFN-alpha/beta R1
Long Name
Interferon alpha/beta Receptor 1
Alternate Names
IFN-aR1, IFN-bR1, IFNAR1, IFNbR1
Gene Symbol
IFNAR1
Additional IFN-alpha/beta R1 Products
Product Documents for IFN-alpha/beta R1 Antibody (SR45-08)
Product Specific Notices for IFN-alpha/beta R1 Antibody (SR45-08)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...