IFT122 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97508

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IFT122 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human IFT122 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for IFT122 Antibody

Immunocytochemistry/ Immunofluorescence: IFT122 Antibody [NBP2-97508]

Immunocytochemistry/ Immunofluorescence: IFT122 Antibody [NBP2-97508]

Immunocytochemistry/Immunofluorescence: IFT122 Antibody [NBP2-97508] - Immunofluorescence staining of IFT122 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human IFT122 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: IFT122 Antibody [NBP2-97508]

Immunohistochemistry-Paraffin: IFT122 Antibody [NBP2-97508]

Immunohistochemistry-Paraffin: IFT122 Antibody [NBP2-97508] - Immunochemical staining of human IFT122 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Applications for IFT122 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFT122

IFT122 is a gene that codes for a protein necessary for cilia formation during neuronal patterning, as it recruits TULP3 to primary cilia and works as a negative regulator of Shh signaling. IFT122 has five isoforms, with lengths of 1241, 1242, 1182, 1131, and 1292 amino acids and weights of 142, 142, 135, 129, and 147 kDa respectively. Current studies are being done on several diseases and disorders including Japanese spotted fever, endemic typhus, vasomer rhinitis, trigeminal neuralgia, synostosis, brachydactyly, paraplegia, neuronitis, and hepatitis. IFT122 has also been shown to have interactions with UBC, IQCB1, and ORF.

Alternate Names

intraflagellar transport 122 homolog (Chlamydomonas), intraflagellar transport protein 122 homolog, SPGWD repeat-containing protein 140, WD repeat domain 10, WD repeat-containing protein 10, WDR10WDR10p, WDR140CED

Gene Symbol

IFT122

Additional IFT122 Products

Product Documents for IFT122 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IFT122 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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